Intermediate

Part:BBa_K3584001:Design

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_United   (2020-10-17)


His tag-Lac operate-T7 tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 136
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add His-tag, lac operator, and T7 tag in the sequence.


Source

It comes from the backbone of the pET28a plasmid.

References