Intermediate
Part:BBa_K3584001:Design
Designed by: Kong Yangyang Group: iGEM20_Shanghai_United (2020-10-17)
His tag-Lac operate-T7 tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 136
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add His-tag, lac operator, and T7 tag in the sequence.
Source
It comes from the backbone of the pET28a plasmid.